TUMOR BIOLOGY SECTION

Prof.Dr. Ruy Gastaldoni Jaeger

Professor
Ph.D.: 1988
E-mail

D.D.S. (1980, University of Sao Paulo), M.S.D. (1983) and Ph.D. (1988) in oral pathology (University of Sao Paulo). Postdoctoral research in cell biology at the NIH (Bechara Kachar's Lab, NIDCD, NIH, USA) from 1991 to 1994. Joined the Institute of Biomedical Sciences in 2001.

Lab tour

CV (Lattes database)

Staff

Our Laboratory studies tumor from different tissues and organs. Our cell lines are from squamous cell carcinoma, adenocarcinoma from breast and salivary glands, and fibrosarcoma

Left: Pleomorphic adenoma cells stained with hematoxylin-eosin (HE). Right:Laminin molecule featuring its cruciform structure made up of 3 chains (α, β and γ) and some of its bioactive peptides.

We are currently investigating the role played by laminin and its bioactive peptide on tumor biology. Laminin is an important extracellular matrix protein, regulating cell growth and differentiation. Our studies are carried out with human cells derived from benign and malignant tumors. We have demonstrated that these cell lines are regulated by laminin and its derived peptides. These particular domains of laminin molecule are important for cell growth and differentiation, protease activity and invadopodia formation. We believe that our system would provide important information on tumor biology. Learn more about our work by clicking here.

Our samples are studied by morphologycal and biochemical methods.

Our projects are funded by São Paulo Research Foundation (FAPESP) and Brazilian National Council for Scientific and Technological Development (CNPq). This laboratory collaborates with INFABIC (National Institute of Photonics Applied to Cell Biology, UNICAMP,SP, Brazil).

• Siqueira AS, Carvalho MR, Monteiro AC, Freitas VM, Jaeger RG, Pinheiro JJ. Matrix metalloproteinases, TIMPs and growth factors regulating ameloblastoma behaviour.. Histopathology 57:128-37, 2010.
• Siqueira AS, Gama-de-Souza LN, Arnaud MV, Pinheiro JJ, Jaeger RG. Laminin-derived peptide AG73 regulates migration, invasion, and protease activity of human oral squamous cell carcinoma cells through syndecan-1 and beta1 integrin.. Tumour Biol. 31:46-58, 2010.
• Nascimento CF, Gama-De-Souza LN, Freitas VM, Jaeger RG Role of MMP9 on invadopodia formation in cells from adenoid cystic carcinoma. Study by laser scanning confocal microscopy. . Microsc Res Tech. 73:99-108, 2010.
• Valois CR, Nunes ES, Jaeger RG, Lima EC, Morais PC, Azevedo RB. Expression patterns of cell adhesion molecules in mice's lung after administration of meso-2,3-dimercaptosuccinic acid-coated maghemite nanoparticles. . J Nanosci Nanotechnol. 9:2846-55, 2009.
• Gama-de-Souza LN, Cyreno-Oliveira E, Freitas VM, Melo ES, Vilas-Boas VF, Moriscot AS, Jaeger RG. Adhesion and protease activity in cell lines from human salivary gland tumors are regulated by the laminin-derived peptide AG73, syndecan-1 and beta1 integrin. . Matrix Biol. 27:402-19, 2008.
• Jaeger RG, Scarabotto-Neto N, Azambuja N Jr, Freitas VM. Secretion of collagen I and tenascin is modulated by laminin-111 in 3D culture of human adenoid cystic carcinoma cells. Int J Exp Pathol. 89:98-105, 2008.
• Freitas VM, Rangel M, Bisson LF, Jaeger RG, Machado-Santelli GM. The geodiamolide H, derived from Brazilian sponge Geodia corticostylifera, regulates actin cytoskeleton, migration and invasion of breast cancer cells cultured in three-dimensional environment. J Cell Physiol. 216:583-94, 2008.
• Freitas VM, Vilas-Boas VF, Pimenta DC, Loureiro V, Juliano MA, Carvalho MR, Pinheiro JJ, Camargo AC, Moriscot AS, Hoffman MP, Jaeger RG. SIKVAV, a laminin alpha1-derived peptide, interacts with integrins and increases protease activity of a human salivary gland adenoid cystic carcinoma cell line through the ERK 1/2 signaling pathway . Am J Pathol. 171:124-38, 2007.
• Morais Freitas V, Nogueira da Gama de Souza L, Cyreno Oliveira E, Furuse C, Cavalcanti de Araújo V, Gastaldoni Jaeger R. Malignancy-related 67kDa laminin receptor in adenoid cystic carcinoma. Effect on migration and beta-catenin expression. . Oral Oncol. 43:987-98, 2007.
• Silva HS, Martins ML, Vilela MJ, Jaeger R, Kachar B. 1/f ruffle oscillations in plasma membranes of amphibian epithelial cells under normal and inverted gravitational orientations Physical Review E 74: 041903, 2006.
• Freitas VM, Scheremeta B, Hoffman MP, Jaeger RG. Laminin-1 and SIKVAV a laminin-1-derived peptide, regulate the morphology and protease activity of a human salivary gland adenoid cystic carcinoma cell line . Oral Oncol. 40:483-9, 2004.
• Pinheiro JJ, Freitas VM, Moretti AI, Jorge AG, Jaeger RG. Local invasiveness of ameloblastoma. Role played by matrix metalloproteinases and proliferative activity. Histopathology 45:65-72, 2004.
• Freitas VM, Jaeger RG. The effect of laminin and its peptide SIKVAV on a human salivary gland adenoid cystic carcinoma cell line. Virchows Archiv 441: 569-576, 2002.
• Araújo V, Sousa S, Jaeger M, Jaeger R, Loyola A, Crivelini M, Araújo N. Characterization of the cellular component of polymorphous low-grade adenocarcinoma by immunohistochemistry and electron microscopy. European J. Cancer (Oral Oncology) 35: 164-172, 1999.

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