André Passaglia Schuch

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[[Image:Foto_Andre.jpg|100px]] [[Image:Foto_Andre.jpg|100px]]
-Possui graduação em Ciências Biológicas pela Universidade Federal de Santa Maria (2005), onde foi bolsista do Programa Especial de Treinamento (PET-BIO/UFSM) e estagiário voluntário do Centro Regional Sul de Pesquisas Espaciais (CRS/INPE-MCT). Obteve o título de Doutor em 2009, pelo Departamento Interunidades em Biotecnologia do Instituto de Ciências Biomédicas da Universidade de São Paulo. Atualmente é pós-doutorando do Departamento de Microbiologia do Instituto de Ciências Biomédicas da Universidade de São Paulo. Tem experiência nas áreas de Fotobiologia e Reparo de DNA, com ênfase em Biomedicina, Biologia Molecular, Genética e Biofísica, atuando principalmente nos seguintes temas: Mutagênese Ambiental e Biotecnologia.+Degree in Biological Sciences at the Universidade Federal de Santa Maria (2005), where he was a fellow of the Special Training Program (PET-BIO/UFSM) and volunteer at the Southern Regional Space Research (CRS / INPE-MCT). He earned his Ph.D. in 2009 at the Department of Biotechnology of Institute of Biomedical Sciences, University of Sao Paulo. He is currently a postdoctoral fellow at the DNA Repair Lab, Department of Microbiology at USP. He has experience in the areas of photobiology and DNA repair, with emphasis on Biomedicine, Molecular Biology, Genetics and Biophysics, acting on the following topics: Biotechnology and Environmental Mutagenesis.
== Resumo do Projeto == == Resumo do Projeto ==
-Devido a sua localização geográfica, altas doses de radiação ultravioleta (UV) incidem no território brasileiro, onde a população tem como costume se expor prolongadamente aos raios solares. Consequentemente, o uso de produtos contendo filtros solares tornou-se a principal estratégia de proteção adquirida por grande parte da população. A avaliação atual do Fator de Proteção Solar (FPS) de uma substância é aqui questionada devido a verificação de uma eficiente indução de lesões de DNA pela radiação UVA, na qual não é considerada em testes de FPS por não induzir eritema. Desta forma, com o uso da tecnologia Dosímetro de DNA, pretendemos avaliar a eficiência de proteção biológica de substâncias através da determinação do fator de proteção solar ao DNA (FPS-DNA).Os valores do FPS-DNA serão obtidos através da quantificação de lesões de DNA com o uso de enzimas de reparo de DNA e anticorpos específicos, após exposições às radiações UVB, UVA e solar. Esses serão correlacionados com a indução de lesões de DNA e de apoptose em linhagens de fibroblastos humanos (proficientes e deficientes em reparo de DNA). Também, pretende-se construir uma nova linhagem de fibroblasto humano deficiente nas vias de reparo por excisão de nucleotídeos (mutada no gene XPA) e no reparo por excisão de bases, através do silenciamento do gene OGG1 com o uso de lentivírus recombinante e transdução do shRNA específico para esse gene. Com essas células, será possível avaliar o real impacto biológico das lesões oxidativas formadas pela luz UVA. Portanto, a aplicação do Dosímetro de DNA fornecerá uma análise a nível molecular da qualidade de proteção de uma determinada substância.+Evaluation of photoprotection and determination of DNA lesions induced by UV light in the DNA molecule and in human cells
 + 
 +Due to its geographical location, high doses of ultraviolet radiation (UV) reaches on Brazilian territory. Consequently, the use of products containing sunscreens became the main strategy for the protection acquired by the majority of population. Current evaluation of Sun Protection Factor (SPF) of a substance is questioned here because the verification of an efficient induction of DNA lesions by UVA radiation, which is not considered in testing for FPS because it does not induce erythema. Thus, through the use of DNA-dosimeter technology, we intend to evaluate the efficiency of protection of biological substances by determining the sun protection factor to DNA (DNA-FPS). The values of the FPS-DNA will be obtained through the quantification of DNA lesions with the use of DNA repair enzymes and specific antibodies after exposure to UVB radiation, UVA and solar radiation. These data will be also correlated with the induction of DNA lesions and apoptosis in different human fibroblasts cell lines (proficient and deficient in DNA repair). Also, we intend to build a new strain of human fibroblasts deficient in pathways of nucleotide excision repair (mutated at XPA gene) and base excision repair by silencing the OGG1 gene using recombinant lentivirus and transduction of the specific shRNA for this gene. With these cells, it will be possible to assess the real biological impact of oxidative lesions formed by UVA light. Therefore, the application of DNA Dosimeter provide an analysis at the molecular level of quality of protection of a particular substance.
==Publicações== ==Publicações==

Revision as of 17:16, 13 October 2011

en:André Passaglia Schuch

Degree in Biological Sciences at the Universidade Federal de Santa Maria (2005), where he was a fellow of the Special Training Program (PET-BIO/UFSM) and volunteer at the Southern Regional Space Research (CRS / INPE-MCT). He earned his Ph.D. in 2009 at the Department of Biotechnology of Institute of Biomedical Sciences, University of Sao Paulo. He is currently a postdoctoral fellow at the DNA Repair Lab, Department of Microbiology at USP. He has experience in the areas of photobiology and DNA repair, with emphasis on Biomedicine, Molecular Biology, Genetics and Biophysics, acting on the following topics: Biotechnology and Environmental Mutagenesis.


Resumo do Projeto

Evaluation of photoprotection and determination of DNA lesions induced by UV light in the DNA molecule and in human cells

Due to its geographical location, high doses of ultraviolet radiation (UV) reaches on Brazilian territory. Consequently, the use of products containing sunscreens became the main strategy for the protection acquired by the majority of population. Current evaluation of Sun Protection Factor (SPF) of a substance is questioned here because the verification of an efficient induction of DNA lesions by UVA radiation, which is not considered in testing for FPS because it does not induce erythema. Thus, through the use of DNA-dosimeter technology, we intend to evaluate the efficiency of protection of biological substances by determining the sun protection factor to DNA (DNA-FPS). The values of the FPS-DNA will be obtained through the quantification of DNA lesions with the use of DNA repair enzymes and specific antibodies after exposure to UVB radiation, UVA and solar radiation. These data will be also correlated with the induction of DNA lesions and apoptosis in different human fibroblasts cell lines (proficient and deficient in DNA repair). Also, we intend to build a new strain of human fibroblasts deficient in pathways of nucleotide excision repair (mutated at XPA gene) and base excision repair by silencing the OGG1 gene using recombinant lentivirus and transduction of the specific shRNA for this gene. With these cells, it will be possible to assess the real biological impact of oxidative lesions formed by UVA light. Therefore, the application of DNA Dosimeter provide an analysis at the molecular level of quality of protection of a particular substance.

Publicações

Yagura, T, Makita, K, Yamamoto H, Menck, CFM, Schuch, AP (2011) Biological Sensors for Solar Ultraviolet Radiation. Sensors, 11(4), 4277-4294.
Schuch AP, Menck CF (2010)The genotoxic effects of DNA lesions induced by artificial UV-radiation and sunlight. J Photochem Photobiol B; 99(3): 111-116.
Schuch, AP, Galhardo, RS, Lima-Bessa, KM, Schuch, NJ and Menck, CF (2009) Development of a DNA-dosimeter system for monitoring the effects of solar ultraviolet radiation. Photochem. Photobiol. Sci., 8: 111-120. Pubmed
SCHUCH, André Passaglia; GUARNIERI, Ricardo A; ROSA, Marcelo Barcelos; PINHEIRO, Damaris K; MUNAKATA, Nobuo; SCHUCH, Nelson Jorge. Comparisons of Biologically Effective Doses of Solar UV-Radiation Determined with Spore Dosimetry and Spectral Photometry in 2000 – 2003 at Southern Brazil. Advances in Space Research, vol. 37, 1784-1788, 2006.

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