André Passaglia Schuch
From DNA Repair Lab
| Degree in Biological Sciences at the Universidade Federal de Santa Maria (2005), where he was a fellow of the Special Training Program (PET-BIO/UFSM) and volunteer at the Southern Regional Space Research (CRS / INPE-MCT). He earned his Ph.D. in 2009 at the Department of Biotechnology of Institute of Biomedical Sciences, University of Sao Paulo. He is currently a postdoctoral fellow at the DNA Repair Lab, Department of Microbiology at USP. He has experience in the areas of photobiology and DNA repair, with emphasis on Biomedicine, Molecular Biology, Genetics and Biophysics, acting on the following topics: Biotechnology and Environmental Mutagenesis. |
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[edit] Research ProjectEvaluation of photoprotection and determination of DNA lesions induced by UV light in the DNA molecule and in human cells Due to its geographical location, high doses of ultraviolet radiation (UV) reaches on Brazilian territory. Consequently, the use of products containing sunscreens became the main strategy for the protection acquired by the majority of population. Current evaluation of Sun Protection Factor (SPF) of a substance is questioned here because the verification of an efficient induction of DNA lesions by UVA radiation, which is not considered in testing for FPS because it does not induce erythema. Thus, through the use of DNA-dosimeter technology, we intend to evaluate the efficiency of protection of biological substances by determining the sun protection factor to DNA (DNA-FPS). The values of the FPS-DNA will be obtained through the quantification of DNA lesions with the use of DNA repair enzymes and specific antibodies after exposure to UVB radiation, UVA and solar radiation. These data will be also correlated with the induction of DNA lesions and apoptosis in different human fibroblasts cell lines (proficient and deficient in DNA repair). Also, we intend to build a new strain of human fibroblasts deficient in pathways of nucleotide excision repair (mutated at XPA gene) and base excision repair by silencing the OGG1 gene using recombinant lentivirus and transduction of the specific shRNA for this gene. With these cells, it will be possible to assess the real biological impact of oxidative lesions formed by UVA light. Therefore, the application of DNA Dosimeter provide an analysis at the molecular level of quality of protection of a particular substance. [edit] PublicaçõesYagura, T, Makita, K, Yamamoto H, Menck, CFM, Schuch, AP (2011) Biological Sensors for Solar Ultraviolet Radiation. Sensors, 11(4), 4277-4294.
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