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From DNA Repair Lab

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-[[Image:ner scheme.jpg|thumb|200px|right|Scheme representing the different steps for nucleotide excision repair]]]+[[pt-br:Pesquisa]]
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 +[[Image:ner scheme.jpg|thumb|200px|right|Scheme representing the different steps of nucleotide excision repair]]
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-'''''Main recent publications''''' ([[Publications|complete list]]):+'''''Main recent publications''''' [http://www.icb.usp.br/~mutagene/index_pt-br.php/Publica%C3%A7%C3%B5es complete list]:
 + 
 +01. Moraes, MCS, Cabral-Neto, JB, Menck, CFM (2012) [http://www.bioscience.org/2012/v17/af/3992/list.htm DNA repair mechanisms protect our genome from carcinogenesis.] Frontiers in Bioscience, 17: 1362-1388.
 + 
 +02. Moraes MC, Andrade AQ, Carvalho H, Guecheva T, Agnoletto MH, Henriques JA, Sarasin A, Stary A, Saffi J, Menck CF (2012) [http://www.sciencedirect.com/science/article/pii/S0304383511005659 Both XPA and DNA polymerase eta are necessary for the repair of doxorubicin-induced DNA lesions.] Cancer Lett. 314: 108-118. ([http://www.sciencedirect.com/science?_ob=MiamiImageURL&_cid=271149&_user=5674931&_pii=S0304383511005659&_check=y&_origin=article&_zone=toolbar&_coverDate=01-Jan-2012&view=c&originContentFamily=serial&wchp=dGLbVBA-zSkzV&md5=64340c79233ebb71d5df194b94ab2ff8/1-s2.0-S0304383511005659-main.pdf PDF])
 + 
 +03. Schuch AP, Menck CF (2010) [http://www.sciencedirect.com/science/article/pii/S1011134410000679 The genotoxic effects of DNA lesions induced by artificial UV-radiation and sunlight. J Photochem Photobiol B; 99(3): 111-116.]([http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6TH0-4YMPX7D-1-C&_cdi=5268&_user=5674931&_pii=S1011134410000679&_origin=&_coverDate=06%2F01%2F2010&_sk=999009996&view=c&wchp=dGLbVlW-zSkzS&md5=a65c5655b5b8d94d116fc41da8c0c325&ie=/sdarticle.pdf PDF])
-01. Menck, CFM (2002) Shining a light on photolyases. Nature Genetics, News and Views, 32: 338-339.+04. Cutiño-Jiménez AM, Martins-Pinheiro M, Lima WC, Martín-Tornet A, Morales OG, Menck CFM (2010) [http://www.sciencedirect.com/science/article/pii/S1055790309003832 Evolutionary placement of Xanthomonadales based in conserved protein signature sequences. Molecular Phylogenetics and Evolution, 54: 524-534.]([http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WNH-4X9TTVC-2-V&_cdi=6963&_user=5674931&_pii=S1055790309003832&_origin=&_coverDate=02%2F28%2F2010&_sk=999459997&view=c&wchp=dGLzVzz-zSkWz&md5=2a786750a6dbe2a249ee337bd62463a4&ie=/sdarticle.pdf PDF])
-02. Costa RM, Chiganças V, Galhardo RS, Carvalho H, Menck CFM (2003) The eukaryotic nucleotide excision repair pathway. Biochimie, 85: 1083-1099.+05. Batista LF, Roos WP, Kaina B and Menck CF (2009) [http://mcr.aacrjournals.org/content/7/2/237.full p53 mutant human glioma cells are sensitive to UV-C induced apoptosis due to impaired CPD removal. Molecular Cancer Research 7(2): 237-246.]([http://mcr.aacrjournals.org/content/7/2/237.full.pdf+html PDF])
-03. Chiganças V, Sarasin, A and Menck, CF (2004) CPD-photolyase adenovirus-mediated gene transfer in normal and DNA repair deficient human cells. Journal Cell Science 117: 3579-3592.+06. Leite, RA, Marchetto, MC, Muotri, AC, Vasconcelos, DM, Oliveira, ZNP, Machado MCR , Menck, CF (2009) [http://www.nature.com/jid/journal/v129/n2/full/jid2008239a.html Identification of XP complementation groups by recombinant adenovirus carrying DNA repair genes. Journal Investigative Dermatology, 129(2):502-506.]([http://www.nature.com/jid/journal/v129/n2/pdf/jid2008239a.pdf PDF])
-04. Marchetto MCN*, Muotri AR*, Burns DK, Friedberg EC and Menck CFM (2004) Gene transduction in skin cells: preventing cancer in xeroderma pigmentosum mice. Proc. Natl. Acad. Sci. USA 101 (51): 17759-17764. Both authors have equally contributed.+07. Lima WC, Varani AM and Menck CF (2009) [http://mbe.oxfordjournals.org/content/26/2/399.full NAD biosynthesis evolution in bacteria: lateral gene transfer of kynurenine pathway in Xanthomonadales and Flavobacteriales. Molecular Biology of Evolution, 26(2): 399-406.]([http://mbe.oxfordjournals.org/content/26/2/399.full.pdf+html PDF])
-05. Lima-Bessa, KM and Menck, CFM (2005) Skin cancer: lights on genome lesions. Current Biology, 15(2): R58-R61.+08. Batista LF, Kaina B, Meneghini R, Menck CF (2009) [http://www.sciencedirect.com/science/article/pii/S1383574208001361 How DNA lesions are turned into powerful killing structures: Insights from UV-induced apoptosis. Mutat Res Reviews, 681(2-3):197-208.]([http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6T2G-4TGHNJX-2-7&_cdi=4918&_user=5674931&_pii=S1383574208001361&_origin=&_coverDate=06%2F30%2F2009&_sk=993189997&view=c&wchp=dGLzVzb-zSkzV&md5=8199043bcbbd880bb707f209988c93ca&ie=/sdarticle.pdf PDF])
-06. Armelini MG, Muotri AR, Marchetto MCN, Lima-Bessa KM, Sarasin A and Menck CFM (2005) Restoring DNA repair capacity of cells from three distinct diseases by XPD gene recombinant adenovirus. Cancer Gene Therapy 12(4): 389-396.+09. Lima-Bessa KM, Armelini MG, Chiganças V, Jacysyn JF, Amarantes-Mendes GP, Sarasin A and Menck CFM (2008) [http://www.sciencedirect.com/science/article/pii/S1568786407003801 CPDs and 6-4PPs play different roles in UV-induced cell death in normal and NER-deficient human cells, DNA Repair 7(2): 303-312.]([http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6X17-4RDB91D-1-F&_cdi=7235&_user=5674931&_pii=S1568786407003801&_origin=&_coverDate=02%2F01%2F2008&_sk=999929997&view=c&wchp=dGLbVlW-zSkzk&md5=9272ebe8d2d64fc1be9e9d0ca3ad97a7&ie=/sdarticle.pdf PDF])
-07. Galhardo, RS, Rocha, R. Marques, MV and Menck, CF (2005) A SOS-regulated operon involved in damage-inducible mutagenesis in Caulobacter crescentus. Nucleic Acids Research 33 (8): 2603-2614.+10. Marchetto MCN*, Muotri AR*, Burns DK, Friedberg EC and Menck CFM (2004) [http://www.pnas.org/content/101/51/17759.full Gene transduction in skin cells: preventing cancer in xeroderma pigmentosum mice. Proc. Natl. Acad. Sci. USA 101 (51): 17759-64. Both authors have equally contributed.]([http://www.pnas.org/content/101/51/17759.full.pdf+html PDF])
-08. Batista LFZ, Chiganças V, Brumatti G, Amarante-Mendes GP and Menck CFM (2006) Involvement of DNA replication in ultraviolet-induced apoptosis of mammalian cells. Apoptosis 11(7): 1139-1148.+11. Costa RM, Chiganças V, Galhardo RS, Carvalho H, Menck CFM (2003) [http://www.sciencedirect.com/science/article/pii/S0300908403001950 The eukaryotic nucleotide excision repair pathway. Biochimie, 85: 1083-1099.]([http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6VRJ-4B5503G-2-9&_cdi=6236&_user=5674931&_pii=S0300908403001950&_origin=&_coverDate=11%2F30%2F2003&_sk=999149988&view=c&wchp=dGLbVzW-zSkWz&md5=9b6b53c0809f91cde57da87249e21d36&ie=/sdarticle.pdf PDF])
-09. Lima-Bessa K, Chiganças V, Stary A, Kanouche P, Sarasin A, Armelini MG, Jacysyn JF, Amarantes-Mendes GP, Cordeiro-Stone M, Cleaver JE and Menck CFM (2006). Adenovirus mediated transduction of the human DNA polymerase eta cDNA. DNA Repair 5: 925-934.+12. Menck, CFM (2002) [http://www.nature.com/ng/journal/v32/n3/full/ng1102-338.html Shining a light on photolyases. Nature Genetics, News and Views, 32: 338-339.]([http://www.nature.com/ng/journal/v32/n3/pdf/ng1102-338.pdf PDF])

Current revision

Scheme representing the different steps of nucleotide excision repair
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Scheme representing the different steps of nucleotide excision repair

DNA Repair Lab


Most of our research activities are based on the understanding of DNA repair and mutagenesis mechanisms in living cells. Several of our approaches include studies on mammalian (basically human) cells in culture, where we try to learn the mechanisms of DNA damage tolerance and removal and cell death (apoptosis) signalling in DNA repair deficient cells (most from the human disease xeroderma pigmentosum). We have developed several recombinant viral vectors (mainly derived from adenovirus), carrying DNA repair genes, which are used in these studies. These vectors are also been used directly in vivo, by infecting DNA repair mice strains, making our lab in the trail for Gene Therapy protocols targeted for skin diseases. Recently, we are developing systems to knock-down DNA repair genes with RNA interference in other to investigate the mechanisms of the interaction of different DNA repair processes. With the recent enormous amount of data generated by genomic approaches, and our own work in genome projects, we have approached the analysis of genome evolution, by using bioinformatics tools. In fact, DNA repair genes are highly conserved and highly appropriated for these studies, as indicated by our work with DNA repair genes in plants. Moreover, based on the knowledge of the complete genome sequence of Caulobacter crescentus, we intend to identify and investigate the genes involved in the DNA repair of this alpha proteobacteria. This bacterial model has been highly useful in studies of cell differentiation and cell cycle in prokaryotes and the possibility to synchronize these bacteria may be useful to ascertain the effect of cell cycle on the repair functions in these cells.


Main recent publications complete list:

01. Moraes, MCS, Cabral-Neto, JB, Menck, CFM (2012) DNA repair mechanisms protect our genome from carcinogenesis. Frontiers in Bioscience, 17: 1362-1388.

02. Moraes MC, Andrade AQ, Carvalho H, Guecheva T, Agnoletto MH, Henriques JA, Sarasin A, Stary A, Saffi J, Menck CF (2012) Both XPA and DNA polymerase eta are necessary for the repair of doxorubicin-induced DNA lesions. Cancer Lett. 314: 108-118. (PDF)

03. Schuch AP, Menck CF (2010) The genotoxic effects of DNA lesions induced by artificial UV-radiation and sunlight. J Photochem Photobiol B; 99(3): 111-116.(PDF)

04. Cutiño-Jiménez AM, Martins-Pinheiro M, Lima WC, Martín-Tornet A, Morales OG, Menck CFM (2010) Evolutionary placement of Xanthomonadales based in conserved protein signature sequences. Molecular Phylogenetics and Evolution, 54: 524-534.(PDF)

05. Batista LF, Roos WP, Kaina B and Menck CF (2009) p53 mutant human glioma cells are sensitive to UV-C induced apoptosis due to impaired CPD removal. Molecular Cancer Research 7(2): 237-246.(PDF)

06. Leite, RA, Marchetto, MC, Muotri, AC, Vasconcelos, DM, Oliveira, ZNP, Machado MCR , Menck, CF (2009) Identification of XP complementation groups by recombinant adenovirus carrying DNA repair genes. Journal Investigative Dermatology, 129(2):502-506.(PDF)

07. Lima WC, Varani AM and Menck CF (2009) NAD biosynthesis evolution in bacteria: lateral gene transfer of kynurenine pathway in Xanthomonadales and Flavobacteriales. Molecular Biology of Evolution, 26(2): 399-406.(PDF)

08. Batista LF, Kaina B, Meneghini R, Menck CF (2009) How DNA lesions are turned into powerful killing structures: Insights from UV-induced apoptosis. Mutat Res Reviews, 681(2-3):197-208.(PDF)

09. Lima-Bessa KM, Armelini MG, Chiganças V, Jacysyn JF, Amarantes-Mendes GP, Sarasin A and Menck CFM (2008) CPDs and 6-4PPs play different roles in UV-induced cell death in normal and NER-deficient human cells, DNA Repair 7(2): 303-312.(PDF)

10. Marchetto MCN*, Muotri AR*, Burns DK, Friedberg EC and Menck CFM (2004) Gene transduction in skin cells: preventing cancer in xeroderma pigmentosum mice. Proc. Natl. Acad. Sci. USA 101 (51): 17759-64. Both authors have equally contributed.(PDF)

11. Costa RM, Chiganças V, Galhardo RS, Carvalho H, Menck CFM (2003) The eukaryotic nucleotide excision repair pathway. Biochimie, 85: 1083-1099.(PDF)

12. Menck, CFM (2002) Shining a light on photolyases. Nature Genetics, News and Views, 32: 338-339.(PDF)

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