Juliana Brandstetter Vilar
From DNA Repair Lab
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| == Research Project == | == Research Project == | ||
| + | ===Evaluation of the repair mechanisms of lesions induced by ACNU=== | ||
| The chemoresistance of tumors is one of the biggest obstacles that commonly lead to failure of therapy. The ability to repair DNA damage and evasion of apoptosis are of great importance, since most of its action is based chemotherapy in the induction of cytotoxicity by the ability to generate lesions in DNA. Thus, an important strategy for improving chemotherapy is the development of more selective approaches and mechanisms to circumvent tumor resistance. In this context, the use of RNAi has emerged as a mechanism able to contribute to the identification of therapeutic targets through elimination of the corresponding mRNA. In this project, we intend to employ innovative techonology of RNAi silencing in the search for potential repair genes previously selected in the basis of its involvement in the repair of induced nimustina (ACNU) ICLs. We intend to test the cytotoxic activity of the chemotherapeutic ACNU challenged with different DNA repair pathways, making sure that this approach is able to sensitize the tumor cells to them. | The chemoresistance of tumors is one of the biggest obstacles that commonly lead to failure of therapy. The ability to repair DNA damage and evasion of apoptosis are of great importance, since most of its action is based chemotherapy in the induction of cytotoxicity by the ability to generate lesions in DNA. Thus, an important strategy for improving chemotherapy is the development of more selective approaches and mechanisms to circumvent tumor resistance. In this context, the use of RNAi has emerged as a mechanism able to contribute to the identification of therapeutic targets through elimination of the corresponding mRNA. In this project, we intend to employ innovative techonology of RNAi silencing in the search for potential repair genes previously selected in the basis of its involvement in the repair of induced nimustina (ACNU) ICLs. We intend to test the cytotoxic activity of the chemotherapeutic ACNU challenged with different DNA repair pathways, making sure that this approach is able to sensitize the tumor cells to them. | ||
Current revision
| Graduated in Biological Sciences (Bachelor of Biomedicine) at Federal University of Goiás (2002) and master's degree in Biology (Area of concentration - Genetics), at the same university (2004). She served as substitute teacher in the courses of Medicine, Veterinary Medicine, Nutrition, Biology and Biomedicine, teaching the disciplines of Genetics and Biostatistics (UFG). She is currently a doctoral student in the postgraduate program in Microbiology at the Institute of Biomedical Sciences, University of Sao Paulo. She has experience in Genetics (with emphasis on Mutagenesis and DNA repair) and Biometry, in the statistical assistance of projects with emphasis on biological data.
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[edit] Research Project[edit] Evaluation of the repair mechanisms of lesions induced by ACNUThe chemoresistance of tumors is one of the biggest obstacles that commonly lead to failure of therapy. The ability to repair DNA damage and evasion of apoptosis are of great importance, since most of its action is based chemotherapy in the induction of cytotoxicity by the ability to generate lesions in DNA. Thus, an important strategy for improving chemotherapy is the development of more selective approaches and mechanisms to circumvent tumor resistance. In this context, the use of RNAi has emerged as a mechanism able to contribute to the identification of therapeutic targets through elimination of the corresponding mRNA. In this project, we intend to employ innovative techonology of RNAi silencing in the search for potential repair genes previously selected in the basis of its involvement in the repair of induced nimustina (ACNU) ICLs. We intend to test the cytotoxic activity of the chemotherapeutic ACNU challenged with different DNA repair pathways, making sure that this approach is able to sensitize the tumor cells to them.
[edit] Main publicationsVILAR, J. B. ; FERRI, P. H.; CHEN CHEN, LEE . Genotoxicity investigation of araticum (Annona crassiflora Mart., 1841, Annonaceae) using SOS-Inductest and Ames test. Brazilian Journal of Biology (Impresso), v. 71, p. 197-202, 2011. VILAR, J. B. ; DOLIVEIRA, M. I. P. ; SANTOS, S. C. ; CHEN CHEN, LEE . Cytotoxic and Genotoxic investigation on Barbatimão [(Stryphnodendron adstringens Mart.). Coville, 1910]. RBCF. Revista Brasileira de Ciências Farmacêuticas (Cessou em 2008. Cont. ISSN 1984-8250 Brazilian Journal of Pharmaceutical Sciences), v. 46, p. 687-694, 2010. VILAR, J. B. ; LEITE, K.R. ; CHEN CHEN, L. . Ginkgo biloba extract (Egb 761) antimutagenicity against mitomycin C and cyclophosphamide in mouse bone marrow. Genetics and Molecular Research, v. 8, p. 328-333, 2009. VILAR, J. B. ; FERREIRA, F.L. ; FERRI, P. H.; GUILLO, L.A.; CHEN CHEN, L. .Assessment of the antimutagenic activity of ethanolic extract of araticum (Annona crassiflora Mart.) by micronucleus test in mice. Brazilian Journal of Biology, v. 68, p. 141-147, 2008. CHIROMATZO. A, O. E.; OLIVEIRA, T.Y.K.; PEREIRA, G. ; COSTA, A. Y. ; MONTESCO, C. A. E. ; GRAS, D. E. ; YOSETAKE, F. ; VILAR, J. B. ; CERVATO, M. ; PRADO, P. R. R. ; CARDENAS, R. G. C. C. L. ; CERRI, R. ; BORGES, R. L. ; SILVA JUNIOR, W. A. . miRNApath: a database of miRNAs, target genes and metabolic pathways. Genetics and Molecular Research, v. 6, p. 859-865, 2007 |
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