Barbara Cortat

From DNA Repair Lab

(Difference between revisions)
Jump to: navigation, search
Revision as of 23:53, 3 July 2007
Barbara Cortat (Talk | contribs)

← Previous diff
Revision as of 23:55, 3 July 2007
Barbara Cortat (Talk | contribs)

Next diff →
Line 3: Line 3:
|[[Image:barbara.jpg|150px|]] |[[Image:barbara.jpg|150px|]]
| |
-Graduated in Biology at University of Sao Paulo (USP) - Brazil. Currently, I am finalizing my graduation project: Subcloning and Expression ''in vitro'' of CPD and (6-4) photolyases.+Graduated in Biology at University of Sao Paulo (USP) - 2007. Currently, I am finalizing my graduation project: Subcloning and Expression ''in vitro'' of CPD and (6-4) photolyases.
|- |-
|colspan="2"| |colspan="2"|

Revision as of 23:55, 3 July 2007

Graduated in Biology at University of Sao Paulo (USP) - 2007. Currently, I am finalizing my graduation project: Subcloning and Expression in vitro of CPD and (6-4) photolyases.

Research abstract

The ultraviolet light induces in the DNA the formation of covalent linkages between adjacent pyrimidines, which provoke structural distortions in the double helix. The most common injuries are pyrimidine dimers, as the cyclobutane pyrimidine (CPD) and the pyrimidine (6-4) pyrimidone photoproducts ((6-4) PP). One mechanisms that can be used by the cells for removal of these injuries is the photoreactivation, a form of direct repair that is characterized for having an only enzyme (photolyase) to remove these injuries using the energy of solar light (300-500 nm). Two types of photolyases had been identified in the nature: a specific one for CPD (CPD-photolyase), and another specific one for (6-4) PP ((6-4) - photolyase). The present work intends to subcloning the genes that codifies for CPD-photolyase e (6-4) - photolyase in bacterial plasmids. These constructions must allow the attainment of good amounts of the respective proteins, whose activity will be tested in vitro. With the attainment of two purified proteins, we will be able to carry through some projects that could identify the relative importance of these two injuries in some deleterious effect of light UV of different wave lengths (UVA, UVB, UVC and solar light).

Publicações

Other carried through works

Participation on the transcriptome sequencing project of Aedes aeqypti , in contribution with national and international laboratories.

Personal tools