Luís Polo

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I am undergraduate biology student in São Paulo university. I develop a Scientific Initiation project,supported by FAPESP, entitled Valuation of a possible synergism between different repair pathways in cisplatin sensibility under orientation of Prof. Dr. Carlos Frederico Martins Menck e co-orientation of Dra. Luciana Nogueira de Sousa Andrade.


The cellular response to DNA damage cover a great variety of different process such as cell cycle arrest, damage tolerance, damage repair or apoptosis. Mutants cell to repair proteins have greater sensibility to genotoxic agents and DNA repair pathways alterations are often involve in chemotherapics chemoresistance. Our objective is to analyse the participation of different repair pathways in response to the chemotherapic agent cisplatin. To achieve this, XP12RO e XP4PA cell lineages – deficient cells in nucleotide excision repair (NER) genes XPA and XPC, respective – will be expose to cisplatin and cytotoxicity will be analysed through clonogenic assay and percentage of hypodiploid cell by flow citometry. Later, siRNA against DNA-PK (DNA dependent kinase, a protein from the non-homologous end joining repair pathway, NHEJ) will be use to explore the NHEJ contribution in cisplatin sensibility.



See also

Nucleotide excision repair (NER)


Non-homologous end joining repair (NHEJ)

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