DNA electroelution

1. Run the restriction enzyme digested DNA in a big well of a 1%
   Agarose gel.

3. Overlay the gel with a small volume of EtBr solution (0.5 ug/ml) for 5-15 min.

3. Cut the desired band over over UV light.

4. Electroelute the agarose piece in a closed dialysis sack immersed in 0.2x TAE
   at 100V for one hour (at least). The dialysis sack should contain 400-500 ul of 0.2x
   TAE.

5. Turn the power supply off.

6. Take the gel out of the sack and close the sack again. Put the sack back 
   into the electrophoresis apparatus. Reverse the current for 30 sec.

7. Extract the contents of the sack with phenol and then phenol-CHCl3 (1:1).

8. Precipitate the aqueous phase with glycogen (1 ul of a 20mg/ml solution) + 0.1 vol
   of NaOAc 3M + 2 vols. of Ethanol.

9. Let it stand overnight at -20 oC or 30 min at -70 oC.

10. Centrifuge at 14,000 rpm for 20 min at 4 oC.

11. Wash with 70% (v/v) Ethanol (at 14,000 rpm/20 min) and dry in a speedvac.

12. Ressuspend the pellet in 20-25 ul of TE.

13. Run 1 ul in a 1% Agarose gel to get an idea of how much you have.