Bradford Protein Determination
1. Done in an ELISA plate and read at 595nm.
2. Dilute standard IgG (1,41 mg/ml):
35,5 ul + 464,5 ul water -> 100 ug/ml
3. Standard graph:
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TUBE IgG Water Bradford IgG
nr (100 ug/ml) Reagent (ug)
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1 20 ul 380 ul 100 ul 2,0
2 40 ul 360 ul 100 ul 4,0
3 60 ul 340 ul 100 ul 6,0
4 80 ul 320 ul 100 ul 8,0
5 100 ul 300 ul 100 ul 10,0
6 150 ul 250 ul 100 ul 15,0
Blank - 400 ul 100 ul 20,0
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OBS.: Don't forget the duplicates for each point!
4. Let stand between 5 min and 1hr at room temperature before reading the reactions.
5. Results:
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TUBE A 595nm | A 595 nm
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1 |
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2 |
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3 |
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4 |
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5 |
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6 |
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Blank |
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